Ex Vivo Phenotype and Frequency of Influenza Virus-Specific CD4 Memory T Cells
Open Access
- 1 July 2004
- journal article
- research article
- Published by American Society for Microbiology in Journal of Virology
- Vol. 78 (13) , 7284-7287
- https://doi.org/10.1128/jvi.78.13.7284-7287.2004
Abstract
Recent advances in class II tetramer staining technology have allowed reliable direct ex vivo visualization of antigen-specific CD4 T cells. In order to define the frequency and phenotype of a prototype response to a nonpersistent pathogen, we have used such techniques to analyze influenza virus-specific memory CD4 T cells directly from blood. These responses are stably detectable ex vivo at low frequencies (range, 0.00012 to 0.0061% of CD4 T cells) and display a distinct “central memory” CD62L+phenotype.Keywords
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