The Tetrahymena ribozyme acts like an RNA restriction endonuclease
- 4 December 1986
- journal article
- Published by Springer Nature in Nature
- Vol. 324 (6096) , 429-433
- https://doi.org/10.1038/324429a0
Abstract
A shortened form of the Tetrahymena self-splicing ribosomal RNA intervening sequence acts as an endoribonuclease, catalysing the cleavage of large RNA molecules by a mechanism involving guanosine transfer. The sequence specificity approaches that of the DNA restriction endonucleases. Site-specific mutagenesis of the enzyme active site alters the substrate sequence specificity in a predictable manner, so that endoribonucleases can be synthesized to cut at a variety of tetranucleotide sequences.Keywords
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