Structure and chromosomal localization of the human 2?,3?-cyclic nucleotide 3?-phosphodiesterase gene
- 1 July 1992
- journal article
- Published by Wiley in Annals of Human Genetics
- Vol. 56 (3) , 243-254
- https://doi.org/10.1111/j.1469-1809.1992.tb01149.x
Abstract
Human brain cDNA clones for the myelin associated enzyme 2′ 3′ ‐cyclic nucleotide 3′ ‐phosphodiesterase (CNPase) have been isolated and sequenced. The only 5′ untranslated region (UTR) sequence found was that of a human CNPII mRNA, with no direct evidence for a CNPI mRNA. Human CNPase cDNAs were used to isolate genomic clones containing the human CNPase gene which is 9 kb long. Four exons were identified, separated by three introns, and the sequence of each exon and intron/exon boundary has been established. The polymerase chain reaction (PCR) was used to detect the presence of the human CNPase gene in DNA from a panel of rodent/human somatic cell hybrids. By this means the human CNPase gene was mapped to chromosome 17. In situ hybridization of a human CNPase genomic clone to metaphase chromosomes further localized this gene to chromosomal band 17q21.Keywords
This publication has 27 references indexed in Scilit:
- Detection of specific sequences among DNA fragments separated by gel electrophoresisPublished by Elsevier ,2006
- Alternative splicing of mouse brain 2′,3′-cyclic-nucleotide 3′-phosphodiesterase mRNABiochemical and Biophysical Research Communications, 1990
- Chromosomal locations of genes encoding 2′,3′ cyclic nucleotide 3′‐phosphodiesterase and glial fibrillary acidic protein in the mouseJournal of Neuroscience Research, 1988
- cDNA cloning and amino acid sequence of human brain 2′,3′-cyclic-nucleotide 3′-phosphodiesteraseBiochemical and Biophysical Research Communications, 1988
- Molecular cloning of the myelin specific enzyme 2′,3′‐cyclic‐nucleotide 3′‐phosphohydrolaseFEBS Letters, 1987
- Human myosin heavy chain genes assigned to chromosome 17 using a human cDNA clone as probeAnnals of Human Genetics, 1985
- Two molecular forms of the isolated brain enzyme 2′,3′‐cyclic nucleotide 3′‐phosphodiesteraseFEBS Letters, 1981
- Purification of Rat 2′,3′‐Cyclic Nucleotide 3′‐PhosphodiesteraseJournal of Neurochemistry, 1981
- PURIFICATION OF 2′,3′‐CYCLIC NUCLEOTIDE 3′‐PHOSPHOHYDROLASE FROM BOVINE BRAIN BY IMMUNOAFFINITY CHROMATOGRAPHY: FURTHER BIOCHEMICAL CHARACTERIZATION OF THE PROTEINJournal of Neurochemistry, 1979
- PURIFICATION AND COMPARISON OF 2′,3′‐CYCLIC NUCLEOTIDE 3′‐PHOSPHOHYDROLASES FROM BOVINE BRAIN AND SPINAL CORDJournal of Neurochemistry, 1978