The Inhibition of Vaccinia Virus Replication by 5,6-Dichloro-1-beta-D-ribofuranosylbenzimidazole (DRB): an Effect at the Assembly Stage

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Abstract
The step sensitive to DRB (5,6-dichloro-1-.beta.-D-ribofuranosylbenzimidazole) in vaccinia virus replication was investigated. DRB (90 .mu.M) extensively inhibited the yield of vaccinia virus after infection of [mouse] Ehrlich ascites tumor cells. DRB did not inhibit cytoplasmic vaccinia DNA replication. Cytoplasmic viral RNA synthesis (early and late) was also apparently unaffected and the virus RNA thus synthesized were normal sized. The expression of early, intermediate and late proteins was not detectably impaired by DRB in vaccinia virus-infected cells. DRB inhibited vaccinia virus replication at the assembly stage since most of the virus DNA remained in a DNase-sensitive form in the infected cells and the virus was not normally coated with virus proteins.