Direct isolation in cell culture of human rotaviruses and their characterization into four serotypes

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Abstract
Of 73 rotavirus-positive [human] fecal specimens tested, 39 yielded a human rotavirus that could be cultivated serially in [rhesus monkey kidney] MA104 and/or primary African green monkey kidney cells; 18 were serotyped. Four distinct serotypes were identified by plaque reduction and/or tube neutralization assay, and 3 of these serotypes were the same as those established previously by plaque reduction, using human rotaviruses cultivated by genetic reassortment with a cultivable bovine rotavirus. Ten human rotavirus strains received from Japan were found to be similar, if not identical, to the candidate prototype strains representing these 4 human rotavirus serotypes.