Restoration of the properties of carnitine palmitoyltransferase I in liver mitochondria during re-feeding of starved rats
- 15 October 1986
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 239 (2) , 485-488
- https://doi.org/10.1042/bj2390485
Abstract
The recovery of the parameters of the kinetic properties of carnitine palmitoyltransferase (CPT) I in liver mitochondria of starved rats was studied after re-feeding animals for various periods of time. There were no significant changes either in the activity of the enzyme at high palmitoyl-CoA concentrations or in the affinity of the enzyme for palmitoyl-CoA, or in the sensitivity of CPT I to malonyl-CoA inhibition after 3 h or 6 h re-feeding. After 24 h re-feeding, both the affinity of the enzyme for palmitoyl-CoA and the activity of the enzyme were still not significantly different from those for the enzyme in mitochondria from 24 h-starved animals. By contrast, the sensitivity of CPT I to malonyl-CoA inhibition was largely, but not fully, restored to that observed in mitochondria from fed rats.This publication has 28 references indexed in Scilit:
- Cycloheximide blocks changes in rat liver carnitine palmitoyltransferase 1 activity in starvationBiochemical Journal, 1984
- Efficient hepatic glycogen synthesis in refeeding rats requires continued carbon flow through the gluconeogenic pathway.Journal of Biological Chemistry, 1984
- Effects of pH on the interaction of substrates and malonyl-CoA with mitochondrial carnitine palmitoyltransferase IBiochemical Journal, 1984
- Sensitivity of carnitine acyltransferase I to malonyl-CoA inhibition in isolated rat liver mitochondria is quantitatively related to hepatic malonyl-CoA concentration in vivoBiochemical Journal, 1982
- The utilization of ketone bodies by the interscapular brown adipose tissue of the ratBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1981
- Regulation of hepatic fatty acid metabolism The activities of mitochondrial and microsomal acyl-CoAsn-glycerol 3-phosphate O-acyltransferase and the concentrations of malonyl-CoA, non-esterified and esterified carnitine, glycerol 3-phosphate, ketone bodies and long-chain acyl-CoA esters in livers of fed or starved pregnant, lactating and weaned ratsBiochemical Journal, 1981
- Effets of fasting, adrenalectomy and streptozotocin‐diabetes on sensitivity of hepatic carnitine acyltransferase to malonyl CoAFEBS Letters, 1981
- Lipogenesis in response to an oral glucose load in fed and starved ratsBioscience Reports, 1981
- The effects of starvation and refeeding on carbohydrate and lipid metabolism in vivo and in the perfused rat liver. The relationship between fatty acid oxidation and esterification in the regulation of ketogenesis.1973
- A simple method for preparation of methyl-labelled (—) carnitineBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1970