Flow Injection Fluoroimmunoassay for Human Transferrin Using a Protein a Immunoreactor
- 1 April 1994
- journal article
- research article
- Published by Taylor & Francis in Analytical Letters
- Vol. 27 (6) , 1067-1074
- https://doi.org/10.1080/00032719408000279
Abstract
A protein A immunoreactor (immobilized protein A) incorporating flow injection technique was used for on-line fluoroimmunoassay of human transferrin. Antibody immobilized on protein A and antibody-antigen complex formation took place in phosphate buffered saline (PBS, PH 7.4). After washing off excess lucifer yellow VS labelled human transferrin, the antibody-antigen complex was eluted with acid buffer and detected. Experimental variables have been studied and the method has been used to determine the transferrin contents in human serum.Keywords
This publication has 10 references indexed in Scilit:
- Flow injection electrochemical enzyme immunoassay for theophylline using a protein A immunoreactor and p-aminophenyl phosphate–p-aminophenol as the detection systemThe Analyst, 1992
- Immunoassay: the ideal trace organic analysis methodPhilosophical Transactions A, 1990
- Dual-column immunoassays using protein G affinity chromatographyAnalytical Chemistry, 1989
- Enzyme-linked flow-injection immunoassay using immobilized secondary antibodiesMicrochimica Acta, 1988
- Rapid heterogeneous competitive electrochemical immunoassay for IgG in the picomole rangeAnalytical Chemistry, 1987
- Electrochemical Sensors in Immunological AnalysisPublished by Springer Nature ,1987
- The Use of Staphylococcal Protein A in Diagnostic VirologyPublished by Springer Nature ,1983
- Lucifer dyes—highly fluorescent dyes for biological tracingNature, 1981
- Cortisol directly determined in serum by fluoroimmunoassay with magnetizable solid phase.Clinical Chemistry, 1980
- A rapid method for the purification of antibody–enzyme conjugatesCanadian Journal of Biochemistry, 1979