Relations between Surface Charge and in Vitro Lysis of Red Blood Cells in Paroxysmal Nocturnal Haemoglobinuria

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Abstract
The effects of neuraminadase on the electrophoretic mobility and in vitro lysis of PNH [paroxysmal nocturnal hemoglobinura], glutathione treated and normal [human] erythrocytes was determined. Neurmanidase strongly affected the in vitro hemolysis of PNH and glutathione treated erythrocytes by substantially elevating the lysis in the Ham acid test while virtually eliminating lysis in the sucrose test. Membrane bound sialic acid or the level of cell surface charge may have a role in the susceptibility of PNH erythrocytes to complement mediated lysis.