Program in BASIC for ferguson plot analysis, using a personal computer: Application to gel electrophoresis in a continuous buffer
- 1 January 1989
- journal article
- research article
- Published by Wiley in Electrophoresis
- Vol. 10 (3) , 199-214
- https://doi.org/10.1002/elps.1150100309
Abstract
A program in BASIC suitable for personal computers is described which is applicable to gel electrophoresis conducted in a single (continuous) buffer. The curve fitting is to a polynomial function, allowing for an objective selection of the most appropriate curve type and order – linear, convex or concave – in the particular application. Results do not differ significantly from previous programs for evaluation of linear Ferguson plots or of curve fitting to an exponential function for evaluating convex plots, executed on mainframe computers such as the DEC-10 (Digital) and IBM 370 computers. Thus, the program combines original versatility with, for the first time, the possibility for widespread application of Ferguson plot analysis on personal computers.Keywords
This publication has 8 references indexed in Scilit:
- Ferguson plots based on absolute mobilities in polyacrylamide gel electrophoresis: Dependence of linearity of polymerization conditions and application to the determination of free mobilityElectrophoresis, 1988
- Linear Ferguson plots of polystyrene sulfate size standards for the quantitative agarose gel electrophoresis of subcellular particlesElectrophoresis, 1988
- Computer simulation of the variable agarose fiber dimensions on the basis of mobility data derived from gel electrophoresis and using the Ogston theoryAnalytical Biochemistry, 1987
- Polystyrene latex particles as size standards in quantitative agarose gel electrophoresis: Application to three plant virusesElectrophoresis, 1987
- Analysis of convex Ferguson plots in agarose gel electrophoresis by empirical computer modelingElectrophoresis, 1986
- Agarose gel electrophoresis of bacteriophages and related particles. III. Dependence of gel sieving on the agarose preparationElectrophoresis, 1983
- Analytical and Preparative Polyacrylamide Gel ElectrophoresisPublished by Springer Nature ,1976
- Estimation of molecular radius, free mobility, and valence using polyacrylamide gel electrophoresisAnalytical Biochemistry, 1971