Isolation of a yeast centromere and construction of functional small circular chromosomes
- 1 October 1980
- journal article
- research article
- Published by Springer Nature in Nature
- Vol. 287 (5782) , 504-509
- https://doi.org/10.1038/287504a0
Abstract
The centromeric DNA (CEN3) from yeast chromosome III was isolated on a 1.6 kbase-pair segment of DNA located near the centromere-linked CDC10 locus of Saccharomyces cerevisiae. When present on a plasmid carrying a yeast chromosomal replicator, CEN3 enables that plasmid to function as a chromosome both mitotically and meiotically. Minichromosomes containing CEN3 are stable in mitosis and segregate as ordinary yeast chromosomes in the 1st and 2nd meiotic divisions.This publication has 21 references indexed in Scilit:
- Sequence of a yeast DNA fragment containing a chromosomal replicator and the TRP1 geneGene, 1980
- Isolation of the centromere-linked CDC10 gene by complementation in yeast.Proceedings of the National Academy of Sciences, 1980
- Isolation and characterisation of a yeast chromosomal replicatorNature, 1979
- Replication in Saccharomyces cerevisiae of plasmid pBR313 carrying DNA from the yeast trpl regionGene, 1979
- High-frequency transformation of yeast by plasmids containing the cloned yeast ARG4 geneProceedings of the National Academy of Sciences, 1979
- High frequency of yeast transformation by plasmids carrying part of entire 2-μm yeast plasmidGene, 1979
- Overlap hybridization screening: Isolation and characterization of overlapping DNA fragments surrounding the leu2 gene on yeast chromosome IIIGene, 1979
- [31] Selection of specific clones from colony banks by screening with radioactive antibodyPublished by Elsevier ,1979
- Transformation of yeast.Proceedings of the National Academy of Sciences, 1978
- Functional expression of cloned yeast DNA in Escherichia coli.Proceedings of the National Academy of Sciences, 1977