Improved Media for Vibrio Cholerae and Salmonella Used in Human Vaccines

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Abstract
Summary A cholera medium has been developed which, mainly due to the joint action of glycerol and phosphate, gives a twelve times higher yield than a simple salt-peptone medium. This medium suits two strains, Ogawa and Inaba, commonly used in vaccine production. Two salmonella media have been designed which give good growth of the following strains employed in vaccines: S. typhi Ty2, 779 and T63; S. paratyphi A, S. paratyphi B and S. paratyphi C. The yield of these two salmonella media is between 5.6 to 7.4 times higher than that of a Bacto nutrient agar medium. This improvement is mainly due to the incorporation of glycerol, phosphate and sodium-glycero-phosphate.