A HIGH MOLECULAR WEIGHT PRECURSOR OF LUTEINIZING HORMONE RELEASING HORMONE FROM RAT HYPOTHALAMUS
- 1 January 1983
- journal article
- research article
- Published by The Endocrine Society in Endocrinology
- Vol. 112 (1) , 390-392
- https://doi.org/10.1210/endo-112-1-390
Abstract
A poly A+ enriched fraction of total RNA extracted from rat hypothalamus was translated in a messenger RNA-dependent cell free system from rabbit reticulocyte lysate supplemented with 3H-leucine. The translation products were immunoprecipitated using a specific antiserum to luteinizing hormone releasing hormone (LHRH). Following sodium dodecyl sulphate polyacrylamide gel electrophoresis and autoradiography of the immunoprecipitates, a single immunoreactive polypeptide was detected with an apparent molecular weight of 28,000. This is approximately twenty times larger than the active peptide,and thus suggests the presence of a high molecular weight precursor for the decapeptide, LHRH.Keywords
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