Permeation of small molecules into the cavity of ferritin as revealed by proton nuclear magnetic resonance relaxation
- 1 April 1995
- journal article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 307 (1) , 253-256
- https://doi.org/10.1042/bj3070253
Abstract
The NMR relaxation technique was used to investigate the permeation of molecules into the cavity of ferritin. Spin-lattice relaxation times in the rotating frame of various probe molecules were measured for solutions of recombinant horse L-apoferritin without iron and horse spleen apoferritin with very small amounts of ferric ions. The results show that molecules larger than the size of the ferritin channels can pass through the channels into the ferritin interior, and that the maximum size of molecules for the permeation is smaller than maltotriose.Keywords
This publication has 12 references indexed in Scilit:
- Heterogeneities in ferritin dimers as characterized by gel filtration, nuclear magnetic resonance, electrophoresis, transmission electron microscopy, and gene engineering techniquesBiochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1994
- Structure, function, and evolution of ferritinsJournal of Inorganic Biochemistry, 1992
- Identification of the iron entry channels in apoferritin. Chemical modification and spectroscopic studiesBiochemistry, 1989
- Release of iron from ferritin molecules and their iron-cores by 3-hydroxypyridinone chelators in vitroJournal of Inorganic Biochemistry, 1989
- FERRITIN: STRUCTURE, GENE REGULATION, AND CELLULAR FUNCTION IN ANIMALS, PLANTS, AND MICROORGANISMSAnnual Review of Biochemistry, 1987
- Iron(III) clusters bound to horse spleen apoferritin: an x-ray absorption and Moessbauer spectroscopy study that shows that iron nuclei can form on the proteinBiochemistry, 1987
- Ferritin: design and formation of an iron-storage moleculePhilosophical Transactions of the Royal Society of London. B, Biological Sciences, 1984
- Mechanism and kinetics of iron release from ferritin by dihydroflavins and dihydroflavin analogsBiochemistry, 1978
- Ferritin iron uptake and release. Structure–function relationshipsBiochemical Journal, 1974
- The determination of iron in plasma or serumBiochemical Journal, 1955