The determination of iron in plasma or serum
- 1 April 1955
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 59 (4) , 599-602
- https://doi.org/10.1042/bj0590599
Abstract
Using Fe59, it was shown that iron is effectively separated from beta1 -globulin in plasma by treatment with dilute HC1 and trichloracetic acid (TCA). A simple and rapid method for the determination of Fe in plasma is described. Two ml of 2[image] - HCl is added to 4 ml of plasma and after stirring, 2 ml of 20% TCA added, the mixture stirred for 45 seconds and centrifuged. The supernatant is added to 0.5 ml of 0.4% 2:2'' dipyridyl plus 2.5 ml saturated sodium acetate plus 2 drops of thioglycollic acid and the color intensity measured in a colorimeter with 520-m[mu] filter.Keywords
This publication has 9 references indexed in Scilit:
- Iron Metabolism in SteatorrheaBlood, 1954
- The determination of iron in blood plasma or serumBiochemical Journal, 1953
- PLASMA IRON AND THE TRANSPORT OF IRON IN THE ORGANISM1952
- FERROKINETICS IN NORMAL PERSONS AND IN PATIENTS HAVING VARIOUS ERYTHROPOIETIC DISORDERS 1Journal of Clinical Investigation, 1951
- PLASMA AND RED CELL IRON TURNOVER IN NORMAL SUBJECTS AND IN PATIENTS HAVING VARIOUS HEMATOPOIETIC DISORDERS 1Journal of Clinical Investigation, 1950
- An Iron-binding Component in Human Blood PlasmaScience, 1946
- Colorimetric Determination of Iron with 2,2'-Bipyridyl and with 2,2',2'-TerpyridylIndustrial & Engineering Chemistry Analytical Edition, 1942
- A method for the estimation of iron in biological materialProceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character, 1930