Expression and analysis of the NS2 protein of influenza A virus
- 1 November 1995
- journal article
- research article
- Published by Springer Nature in Archiv für die gesamte Virusforschung
- Vol. 140 (11) , 2067-2073
- https://doi.org/10.1007/bf01322693
Abstract
Influenza NS2 protein was expressed inSaccharomyces cerevisiae using a copper-inducible promoter. The protein produced had a molecular weight of 13 kDa, was reactive with anti-NS2 antiserum and was localised to the yeast cell nucleus. Two-hybrid analysis identified a direct protein-protein interaction between NS2 and the M2 protein of the virus, involving the C-terminal 163 residues of M1. A filter-binding assay localised the M1 binding region to the C-terminal 70 amino acids of NS2.Keywords
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