Synthesis of the NS 2 nonstructural protein messenger RNA of influenza A viruses occurs in the absence of viral protein synthesis
- 1 September 1991
- journal article
- Published by Springer Nature in Archiv für die gesamte Virusforschung
- Vol. 120 (3-4) , 281-288
- https://doi.org/10.1007/bf01310483
Abstract
The NS 2 messenger RNA (mRNA) of several influenza A viruses was shown to be synthesized in primary transcription. Analysis of in vitro translation products of mRNAs from infected MDCK cells treated with cycloheximide indicated that the NS 2 mRNA in addition to the NS 1 mRNA was synthesized with PR/8, Udorn, and Aichi viruses. The findings indicated that the NS 1 mRNA of these viruses was able to be spliced into the NS 2 mRNA as a primary transcript without viral protein synthesis, although the extent of splicing varied among different virus strains.Keywords
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