Multiplication of Virulent Poliovirus in Capuchin Monkey Kidney Cultures Without Microscopically Observed Cytopathogenicity.

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Abstract
Cultures of trypsinized kidneys of South American capuchin monkeys (Cebus capucina) supported the multiplication of Type 1 polio-viruses, even though no cytopathic changes were observed by microscopic examination of the cultures. The failure to observe cytologic changes was found to be due to the small proportion of cells in the culture capable of supporting the growth of virus. After 4 to 5 tissue culture passages the viruses remained virulent for the monkey brain and spinal cord. No evidence was obtained for the propagation of Type 2 and 3 strains in the capuchin cultures.