Isolation of a Non-Neurotropic Variant of Type I Poliomyelitis Virus

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Abstract
Following the adaptation of Type I, Mahoney strain, poliomyelitis virus to mice, further passages in tissue cultures, mice, and alternate monkey skin to tissue cultures resulted in establishment of 4 passage lines of virus, 3 of which appear to be distinct variants. These strains were designated as LS, LS-a, LS-b and LS-c and are differentiated on the basis of their range of host reactions. LS and LS-a react similarly and are the so-called mouse and monkey spinal variants. LS-b is a mouse cerebral strain and LS-c is a non-neurotropic strain which is avirulent for both mice and monkeys by all routes. These variants have unaltered antigenic and tissue culture characteristics. Preliminary experiments with the LS-c strain have shown it to be capable of inducing good antibody titers and a high degree of resistance to virulent virus challenge in monkeys. There is suggestive evidence that continued skin passages of the LS-c strain may yield a dermal poliomyelitis virus variant.