THE EFFECTS OF NONIDET P40 ON THE FUNCTION OF RAT PERITONEAL MAST CELLS in vitro
Open Access
- 1 January 1981
- journal article
- Published by Wiley in British Journal of Pharmacology
- Vol. 72 (1) , 95-100
- https://doi.org/10.1111/j.1476-5381.1981.tb09110.x
Abstract
1 Treatment of purified rat peritoneal mast cells at 37°C with concentrations of the non-ionic detergent nonidet P40 (NP40) up to 0.005% (v/v) failed to reduce their viability. 2 There was a marked reduction in the histamine releasing capacity of NP40-treated mast cells upon challenge with a variety of selective (adrenocorticotrophic hormone 1–24 (Synacthen), rabbit anti-rat IgE antiserum, adenosine triphosphate (ATP) and the calcium ionophore, A 23187) and non-selective (rabbit anti-rat mast cell antiserum plus complement) histamine liberators. 3 Nonidet P40 (0.005%) was found to reduce the activity of a mast cell membrane ‘ecto-enzyme’, calcium-activated ATPase, by about 45% when present at the time of its assay.Keywords
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