Method for quantitative determination of p‐hydroxybenzyl isothiocyanate in digests of seed meal of Sinapis alba L.

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Abstract
A method has been developed for the quantitative determination of p‐hydroxybenzyl isothiocyanate in enzymic digests of seed meal of white mustard (Sinapis alba L.). The isothiocyanate was treated with sodium hydroxide and the released thiocyanate was determined by use of ferric nitrate reagent. A sample in which the ferric thiocyanate complex was destroyed by addition of mercuric chloride was used as a control for the colorimetric determinations. The yields of isothiocyanate obtained after hydrolysis of p‐hydroxybenzylglucosinolate at different pH have been studied. A relative standard deviation of 0.9% was found when the method was applied to micro‐ quantitities. The simplicity and rapidity of the method and its applicability to small samples make it well suited for use in connexion with plant breeding.