Requirement for protein synthesis during the onset of meiosis in bovine oocytes and its involvement in the autocatalytic amplification of maturation‐promoting factor
- 1 May 1995
- journal article
- research article
- Published by Wiley in Molecular Reproduction and Development
- Vol. 41 (1) , 47-53
- https://doi.org/10.1002/mrd.1080410108
Abstract
The present study was carried out using the method of electrofusion, or treatment with okadaic acid (OA), to determine whether protein synthesis at the onset of culture was required for the meiotic resumption of bovine follicular oocytes. Germinal vesicle breakdown (GVBD) occurred in bovine oocytes at 6 hr after separation from their follicles in vitro. Following this, immature germinal vesicle (GV) oocytes, preincubated for 0,2,4, and 6 hr, were fused to mature oocytes. When immature oocytes, preincubated for 0 hr, were fused to mature oocytes and then cultured for 3 hr in basic medium, GVBD was observed in all fused cells, whereas in the case of cultivation in medium supplemented with the protein synthesis inhibitor (25 μg/ml cycloheximide; CX), 39% of the fused cells possessed an intact GV within their cytoplasm. In immature oocytes preincubated for 4 or 6 hr, however, this proportion was significantly reduced to 7% and 4%, respectively, without protein synthesis after fusion. In addition, the CX‐dependent block of GVBD could be overcome in only 13% of bovine follicular oocytes by the addition of 2 μM OA, although 51% of oocytes which synthesized the protein during the first 6 hr of culture induced GVBD in subsequent culture with CX plus OA. Thus, we conclude that the initiation of GVBD in bovine oocytes requires protein synthesized at the onset of meiosis, which is related to the autocatalytic amplification of the maturation‐promoting factor.Keywords
This publication has 33 references indexed in Scilit:
- Changes in protein synthesis pattern during in vitro maturation of goat oocytesMolecular Reproduction and Development, 1992
- Okadaic acid mimics a nuclear component required for cyclin B-cdc2 kinase microinjection to drive starfish oocytes into M phase.The Journal of cell biology, 1991
- The effects of α‐amanitin and cycloheximide on nuclear progression, protein synthesis, and phosphorylation during bovine oocyte maturation in vitroMolecular Reproduction and Development, 1991
- Combined effects of protein synthesis and phosphorylation inhibitors on maturation of mouse oocytes in vitroMolecular Reproduction and Development, 1990
- Characterization of MPF activation by okadaic acid in Xenopus oocyteCell Differentiation and Development, 1990
- Okadaic acid, a specific protein phosphatase inhibitor, induces maturation and MPF formation in Xenopus laevis oocytesFEBS Letters, 1989
- Effect of cycloheximide upon maturation of bovine oocytesReproduction Nutrition Développement, 1989
- Does autocatalytic amplification of maturation‐promoting factor (MPF) exist in mammalian oocytes?Gamete Research, 1988
- Problems of Oocyte Maturation and the Control of Chromosome Cycles*Development, Growth & Differentiation, 1985
- Induction of maturation in small oocytes from sexually immature mice by fusion with meiotic or mitotic cellsExperimental Cell Research, 1978