Studies involving enzymic phosphorylation. 4. The conversion of d-ribose into d-ribose 5-phosphate by extracts of Escherichia coli

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Abstract
Large scale enzymic phosphorylations of D-ribose by means of a cell-free extract of ribose-grown E. coli were carried out in the presence of adenosine triphosphate. A biosynthetic pentose monophos-phate was isolated from the reaction mixture and partially purified. This biosynthetic material is almost certainly ribose 5-phosphate, as shown by the liberation of ribose after treatment with alkaline phosphatase, and by studies of reducing power, acid stability, periodate oxidation and rate of color development in the orcinol reaction.