THE EFFECTS OF ULTRAVIOLET C ON in vitro NUCLEOSOME ASSEMBLY AND STABILITY
- 1 August 1994
- journal article
- Published by Wiley in Photochemistry and Photobiology
- Vol. 60 (2) , 134-138
- https://doi.org/10.1111/j.1751-1097.1994.tb05079.x
Abstract
The effects of ultraviolet C (UVC) irradiation on nucleosome assembly and its stability were investigated quantitatively using an in vitro nucleosome assembly system comprising a plasmid DNA of pBR322 and core histones isolated from rat ascites hepatoma cells. Nucleosomal formation was estimated by analyzing the resulting DNA supercoils. When UVC-irradiated (3000 J/m2) DNA was used as a substrate for the nucleosome assembly system, the nucleosomal formation efficiency was reduced by half compared with nonirradiated DNA. On the other hand, when the reconstituted nucleosomes (minichromosomes) on the nonirradiated DNA were irradiated with UVC (3000 J/m2), about half each were disrupted and retained. These results indicate that it is difficult for UV-damaged DNA regions to supercoil around the histone octamers to form nucleosomes and that the histone octamers in the UV-damaged nucleosomes tend to be dissociated from DNA.Keywords
This publication has 28 references indexed in Scilit:
- Nucleosome rearrangement in human cells following short patch repair of DNA damaged by bleomycinBiochemistry, 1990
- Base sequence specificity of a monoclonal antibody binding to (6-4)photoproductsMutation Research/DNA Repair, 1990
- Preparation and photochemical properties of polymers containing 1-(4-benzylphenyl)-2-propen-1-one or 1-(4-benzoylphenyl)-2-propen-1-one structural unitsJournal of Photochemistry and Photobiology A: Chemistry, 1990
- UV INDUCED (6‐4) PHOTOPRODUCTS ARE DISTRIBUTED DIFFERENTLY THAN CYCLOBUTANE DIMERS IN NUCLEOSOMESPhotochemistry and Photobiology, 1990
- INDUCTION OF DIRECT AND INDIRECT SINGLE‐STRAND BREAKS IN HUMAN CELL DNA BY FAR‐ AND NEAR‐ULTRAVIOLET RADIATIONS: ACTION SPECTRUM AND MECHANISMSPhotochemistry and Photobiology, 1987
- A nonuniform distribution of excision repair synthesis in nucleosome core DNABiochemistry, 1985
- CONFORMATIONS OF DEOXYDODECANUCLEOTIDES WITH PYRIMIDINE (6‐4) PYRIMIDONE PHOTOADDUCTSPhotochemistry and Photobiology, 1985
- TECHNOLOGICAL EXAMINATION OF LOW‐FIRED TERRACOTTA STATUES FROM AYIA IRINI, KEAArchaeometry, 1982
- DNA SINGLE-STRAND LESIONS DUE TO ‘SUNLIGHT’ AND UV LIGHT: A COMPARISON OF THEIR INDUCTION IN CHINESE HAMSTER AND HUMAN CELLS, AND THEIR FATE IN CHINESE HAMSTER CELLSPhotochemistry and Photobiology, 1978
- Folding of the DNA double helix in chromatin-like structures from simian virus 40.Proceedings of the National Academy of Sciences, 1975