Effects of an altered developmental pattern on amino acid uptake for a protoplast isolate of the fungus Entomophaga aulicae under mass-fermentation conditions

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Abstract
The effects of an altered developmental pattern on amino acid uptake for a protoplast isolate of the fungus Entomophaga aulicae were studied under mass-fermentation conditions. Isolate 655A had previously developed through the hyphal body stage, but subsequently lost this ability (then designated I655B) and then developed only as far as the germination of the late fusion sphere stage. This alteration in developmental pattern was accompanied by a shift in the overall amino acid uptake pattern. For comparative purposes, another isolate (I521) which developed only as far as the germination of the late fusion sphere stage was included. The general amino acid uptake patterns fell into three categories and emphasize the similarity of I521 and I655B: (i) earlier and more complete final utilization by isolates 521 and 655B for glutamine, leucine, aspartic acid, threonine, and methionine, (ii) earlier utilization by isolate 655A with more complete final utilization by isolates 521 and 655B for asparagine and proline, and (iii) earlier utilization by isolates 521 and 655A with more complete final utilization by isolates 521 and 655B for serine and tyrosine. The first detectable utilization of serine coincided with late fusion sphere germination for isolates 521 and 655B. During the rapid increase in hyphal body levels for I655A, all added amino acids (except proline, asparagine, and serine which were first utilized earlier) were utilized for the first time. Except for the timing of the initial day of detectable utilization, the pattern of glucose utilization was similar for all three isolates.