Flocculation tests with electrophoretically separated serum proteins

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Abstract
A modified technique was described, suitable for the prepn. of electrophoretically pure proteins from relatively small volumes of serum. Two human sera were separated, normal (N) and hepatitis (H). The thymol turbidity test as carried out on serum depended partly upon the presence of native phospholipids. In tests on protein fractions with added cephalin, [gamma]-globulin acted as a precipitating agent; N albumin inhibited the reaction and H albumin did not; [alpha] and [beta] fractions were without significant effect. The thymol flocculation test, both with and without added cephalin, was positive with [gamma]-globulin, but was negative with N [gamma]-globulin and with all other fractions. It was not inhibited by other H fractions (N fractions were not tested for inhibition). Corresponding data were presented for the colloidal gold reaction, the cephalin-cholesterol test, and the Takata-Ara reaction. Two formulae were obtained which qualitatively accounted for the behavior of whole sera with the gold reagent. The effect of [alpha]- and [beta]-globulin was different in each test. Thus H [alpha]- and [beta]-globulin flocculated the cephalin-cholesterol and Takata-Ara reagents while both N and H[alpha]- and [beta]-globulins were powerful inhibitors of the gold reaction.