PRECIPITATION OF CALCIUM PHOSPHATE IN THE HISTOCHEMICAL METHOD FOR PHOSPHATASE
Open Access
- 1 March 1953
- journal article
- research article
- Published by SAGE Publications in Journal of Histochemistry & Cytochemistry
- Vol. 1 (2) , 114-122
- https://doi.org/10.1177/1.2.114
Abstract
Ca phosphate, when precipitated by the addition of phosphate to solutions similar in composition to the histochemical substrate mixture, does not show any experimentally demonstrable tendency to supersaturation. The factors which may contribute to false localizations of enzymatic activity its the Ca-CoS method for alkaline phosphatase are not well understood and certainly not amenable to mathematical analysis for the time being. At present, diffusion artifacts do not appear to be an important source of error, provided incubation time is not unduly extended.Keywords
This publication has 8 references indexed in Scilit:
- Histochemical studies of alkaline phosphatases in the tissues of the rat using frozen sections. II. Substrate specificity of enzymes hydrolyzing adenosine‐triphosphate, muscle‐ and yeast‐adenylic acids, and creatine‐phosphate at high pH; the histochemical demonstration of myosin ATP‐aseJournal of Cellular and Comparative Physiology, 1952
- ALKALINE PHOSPHATASE OF CELL NUCLEI1951
- Cytological Demonstration of Alkaline PhosphataseNature, 1950
- PITFALLS IN HISTOCHEMISTRYAnnals of the New York Academy of Sciences, 1950
- BERYLLIUM AND GROWTH .1. BERYLLIUM-INDUCED OSTEOGENIC SARCOMATA1950
- PHOSPHATASE OF RABBIT POLYMORPHONUCLEAR LEUCOCYTESCanadian Journal of Research, 1949
- Buffers in the Range of pH 6.5 to 9.6.Experimental Biology and Medicine, 1946
- Investigations of the Phosphatase Activity in Serum and Organs after Ligation of the Common Bile Duct in DogsActa Physiologica Scandinavica, 1946