Characterization of the 5'-terminal capped structures of late simian virus 40-specific mRNA

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Abstract
32P-labeled, late SV-40-specific RNA was iaolated from infected [African green monkey kidney] CV1 cells and completely degraded with RNase T2 and bacterial alkaline phosphatase. The RNase-resistant material was fractionated 2 dimensionally and further characterized with Penicillium nuclease and nucleotide pyrophosphatase. Two major 5'' termini were identified in late SV-40 RNA, namely, 7-methyl Gppp 2'',6-dimethyl ApUp and 7-methyl Gppp 2'',6-dimethyl Ap 2''-methyl UpUp. Both 5'' termini are present in unfractionated viral RNA and in the separated 16S and 19S species. As both caps differ only in secondary modification, they are probably derived from the same site on the DNA. The relatively higher cap II content of the 16S mRNA may be related to its slower rate of turnover.