A METHOD OF DNA HISTOGRAM ANALYSIS BY COMPUTER AND ITS EVALUATION BY SIMULTANEOUS COMBINATION WITH THYMIDINE-H-3 AUTORADIOGRAPHY

  • 1 January 1983
    • journal article
    • research article
    • Vol. 27  (3) , 163-176
Abstract
A method for determining the fractions of cells in the G1, S and G2 + M phases of the cell life cycle, by quantifying DNA histograms derived from static fluorescense cytophotometry, was evaluated by simultaneous combination with 3H-thymidine autoradiography. DNA histograms were obtained by cytofluorometry on the Feulgen-stained autoradiogrpahs of HeLa cells, and mouse and rat hepatocytes, after DNA labeling with 3H-thymidine. The synthetic histogram determined by sum of discrete normal curves technique was fitted to the experimental data according to a weighted least-squares method by a desk-top computer (HP 85F). The mean relative percent deviations of estimated cell cycle phase fractions from the actual phase fractions determined directly on an autoradiograph was 6.6 .+-. 3.3%.