Identification of albumin as the plasma carrier for zinc absorption by perfused rat intestine

Publisher Website
Google Scholar
Abstract
The isolated vascularly perfused rat intestine exhibits an obligatory need for a protein carrier to absorb Zn. This system is ideal for use as a model to identify the plasma carrier during Zn absorption. Affinity chromatography on Blue Sepharose CL-6B was employed to separate the major serum Zn-binding proteins in the portal effluent of the perfused intestine. It was found that 94% of newly absorbed 65Zn was transported in the portal serum-containing perfusate as an albumin-65Zn complex. The identity of albumin as the plasma carrier was confirmed by polyacrylamide-slab-gel electrophoresis. Albumin may be the plasma protein that is involved in removal of Zn from intestinal-mucosal cells and subsequent transport of the metal in portal blood to the liver.

This publication has 13 references indexed in Scilit: