MECHANISM FOR INDUCTION OF ANTI-DNA ANTIBODIES BY BACTERIAL LIPOPOLYSACCHARIDES IN MICE .2. CORRELATION BETWEEN ANTI-DNA INDUCTION AND POLYCLONAL ANTIBODY-FORMATION BY VARIOUS POLYCLONAL LYMPHOCYTE-B ACTIVATORS

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  • 1 January 1977
    • journal article
    • research article
    • Vol. 119  (6) , 2157-2162
Abstract
The capacity of various polyclonal B [bone marrow-derived] lymphocyte activators (PBA) to induce, in mice, the formation of anti-DNA antibodies was compared with their ability to mediate the release of DNA in circulating blood and to stimulate polyclonal antibody synthesis in vivo. Anti-DNA antibodies or polyclonal antibody synthesis were induced in mice after the injection of at least 10 .mu.g lipopolysaccharide (LPS) from Salmonella typhimurium, 1 mg dextran sulfate (DS) or 2 mg purified protein derivative of tubercle bacteria RT32 (PPD). Smaller quantities of LPS (0.1 .mu.g) or DS (500 .mu.g) were sufficient to cause the release of DNA in circulating blood, whereas PPD was not able to provoke such a release at any concentration used. The association of anti-DNA antibodies with polyclonal antibody synthesis in mice injected with various PBA contrasts with the lack of correlation between the formation of anti-DNA antibodies and the release of measurable amounts of DNA in circulating blood. The induction of anti-DNA antibodies by PBA is probably a consequence of the polyclonal B lymphocyte activation.