INVITRO MALIGNANT TRANSFORMATION OF MOUSE FIBROBLASTS BY NON-K-REGION DIHYDRODIOLS DERIVED FROM 7-METHYLBENZ(A)ANTHRACENE, 7, 12-DIMETHYLBENZ(A)ANTHRACENE, AND BENZO(A)PYRENE

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  • 1 January 1976
    • journal article
    • research article
    • Vol. 36  (6) , 2059-2064
Abstract
The 8,9-dihydrodiols of 7-methylbenz(a)anthracene and 7,12-dimethylbenz(a)anthracene and the 7,8-dihydrodiol of benzo(a)pyrene, which are non-K-region diols with adjacent olefinic double bonds that can be metabolized to diol-epoxides, were more active than the parent hydrocarbons in inducing malignant transformation of M2 mouse fibroblasts. A 4th non-K-region diol, the 9,10-dihydrodiol of benzo(a)pyrene was less active than benzo(a)pyrene. The related K-region dihydrodiols, which lack adjacent olefinic double bonds, and 6-hydroxybenzo(a)pyrene were inactive. 7,8-Dihydrobenzo(a)pyrene, a more potent carcinogen than the 9,10 isomer, induced malignant transformation, but the 9,10 isomer was inactive. Transformed cells with abnormal morphology yielded sarcomas on injection into isologous mice. Treated but morphologically normal cells did not. These results support the role of diols and diol-epoxides in the metabolic activation of polycyclic hydrocarbons.

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