Expression of a Clostridium thermocellum endoglucanase gene in Lactobacillus plantarum
- 1 August 1989
- journal article
- research article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 55 (8) , 2095-2097
- https://doi.org/10.1128/aem.55.8.2095-2097.1989
Abstract
Recombinant plasmid pM25 containing the celE gene of Clostridium thermocellum, which codes for an enzymatically active endoglucanase, was transformed into Lactobacillus plantarum by electroporation. Strains harboring pM25 expressed thermostable endoglucanase, which was found predominantly in the culture medium. Two other plasmids, pGK12 and pSA3, were transformed into L. plantarum, and the stability of each plasmid was evaluated.This publication has 11 references indexed in Scilit:
- A rapid boiling method for the preparation of bacterial plasmidsPublished by Elsevier ,2004
- Application of electroporation for transfer of plasmid DNA to Lactobacillus, Lactococcus, Leuconostoc, Listeria, Pediococcus, Bacillus, Staphylococcus, Enterococcus and PropionibacteriumMolecular Microbiology, 1988
- Conserved reiterated domains in Clostridium thermocellum endoglucanases are not essential for catalytic activityGene, 1988
- Molecular Cloning of Clostridium Thermocellum DNA and the Expression of Further Novel Endo- ,4-glucanase Genes in Escherichia ColiMicrobiology, 1987
- Plasmid profiles and transfer of plasmid-encoded antibiotic resistance in Lactobacillus plantarumApplied and Environmental Microbiology, 1985
- Streptococcus-Escherichia coli shuttle vector pSA3 and its use in the cloning of streptococcal genesApplied and Environmental Microbiology, 1985
- Use of Congo red-polysaccharide interactions in enumeration and characterization of cellulolytic bacteria from the bovine rumenApplied and Environmental Microbiology, 1982
- Nonchromosomal Antibiotic Resistance in Bacteria: Genetic Transformation of Escherichia coli by R-Factor DNAProceedings of the National Academy of Sciences, 1972
- SUPERCOILED CIRCULAR DNA-PROTEIN COMPLEX IN Escherichia coli : PURIFICATION AND INDUCED CONVERSION TO AN OPEN CIRCULAR DNA FORMProceedings of the National Academy of Sciences, 1969
- Transduction of linked genetic characters of the host by bacteriophage P1Virology, 1955