Murine cells expressing an HLA molecule are specifically lysed by HLA-restricted antiviral human T cells
- 1 January 1986
- journal article
- Published by Springer Nature in Nature
- Vol. 319 (6049) , 153-154
- https://doi.org/10.1038/319153a0
Abstract
Class I HLA (histocompatibility locus antigen) molecules are the targets of allospecific cytolytic T lymphocytes (CTL) in graft rejection, and constitute the restricting elements necessary for the interaction between antiviral CTL and virus-infected cells. Cells expressing only one HLA in the absence of other human molecules would provide a remarkable model for studying the function of these molecules. However, HLA+ murine cells transfected with human genes are generally not lysed by allospecific human CTL, and this is ascribed to insufficient HLA expression, lack of human beta 2-microglobulin, alteration of HLA molecules or absence of receptors for human T8 or LFA1 molecules in murine cells. Here we report, for the first time, the specific lysis of virus-infected HLA+ murine cells by HLA-restricted antiviral human CTL. Therefore, these murine cells constitute an excellent model for studying the role of HLA molecules.Keywords
This publication has 10 references indexed in Scilit:
- Recognition of HLA-A2 and -B7 antigens by cloned cytotoxic T lymphocytes after gene transfer into human and monkey, but not mouse, cells.Proceedings of the National Academy of Sciences, 1984
- Recognition of HLA-A2 by Cytotoxic T Lymphocytes After DNA Transfer into Human and Murine CellsScience, 1984
- Efficient transfer of cloned DNA into human diploid cells: protoplast fusion in suspension.Molecular and Cellular Biology, 1984
- HLA-B27, A dominant restricting element in antiviral responses?Immunogenetics, 1984
- Identification of several functional subgroups of HLA-B27 by restriction of the activity of antiviral T killer lymphocytesImmunogenetics, 1984
- Recognition by xenogeneic cytotoxic T lymphocytes of cells expressing HLA-A2 or HLA-B7 after DNA-mediated gene transfer.Proceedings of the National Academy of Sciences, 1983
- Stable transformation of mouse L cells for human membrane T-cell differentiation antigens, HLA and beta 2-microglobulin: selection by fluorescence-activated cell sorting.Proceedings of the National Academy of Sciences, 1983
- Identification of human genomic clones coding the major histocompatibility antigens HLA-a2 and HLA-B7 by DNA-mediated gene transfer.Proceedings of the National Academy of Sciences, 1982
- Expression of human class I histocompatibility antigens at the surface of DNA-transformed mouse L cellsImmunogenetics, 1982
- Biochemical transfer of single-copy eucaryotic genes using total cellular DNA as donorCell, 1978