Hormonal Control of Morphogenesis in Leaf Explants of Perilla frutescens Britton var. crispa Decaisne f. viridi-crispa Makino

Publisher Website
Google Scholar
Abstract
Leaf discs of Perilla frutescens var. crispa f. viridi-crispa were cultured on a defined medium to investigate factors influencing bud and root formation, callus induction, somatic embryogenesis, and floral bud formation. Addition of naphthalene-acetic acid (NAA) to the culture medium caused compact callus whereas 2,4-dichlorophenoxy acetic acid (2,4-D) promoted soft and friable callus formation on the surface of the explants. Benzyladenine, when applied with auxin, suppressed callus and root formation. Somatic embryogenesis occurred, when the explants were first grown on nutrient medium containing 2,4-D and organic elements, and then transferred to the 2,4-D free medium. Treatments with cytokinins, N-phenyl-N'-(4-pyridyl) urea and its derivatives induced bud formation. A low concentration of NAA and naphthoxy-acetic acid promoted bud development. Occasional floral bud formation was observed depending on the original leaf positions on mother plants from which the leaf discs were excised. A gradient of floral bud forming capacity along the stem was noted.