POLYMORPHONUCLEAR NEUTROPHIL IODINATION RESPONSE AS AN ESTIMATE OF DEFECTIVE YEAST OPSONIZATION

  • 1 January 1981
    • journal article
    • research article
    • Vol. 43  (1) , 208-214
Abstract
Polymorphonuclear neutrophil iodide uptake can be used as a measure of [baker''s] yeast opsonization, optimal assay conditions are described for the identification of [baker''s] sera defective in this function. The use of standard normal and defective sera permits correction of inter-assay variations resulting from the use of different cell donors. The iodination assay correlated well (r = 0.74, P < 0.001) with a direct assay of yeast opsonization when both were used to measure this function in a panel of 72 sera. Differences in results between the 2 assay systems for some sera may be explained by a requirement for 2 different surface-associated opsonic molecules in the initiation of neutrophil metabolic activity.