Immunochemical determination of CK-MB isoenzyme in human serum. II. An enzymic approach.
- 1 January 1982
- journal article
- research article
- Published by Oxford University Press (OUP) in Clinical Chemistry
- Vol. 28 (1) , 54-58
- https://doi.org/10.1093/clinchem/28.1.54
Abstract
A novel immunochemical technique for a specific enzymic determination of the myocardial isoenzyme of creatine kinase, CK-MB, involves determination of B-subunit activity of a specimen in which the M-subunit activity has been inhibited by specific antibodies to the M-subunit. Interfering activities from CK-BB isoenzyme, atypical forms of creatine kinase, and adenylate kinase are eliminated by using a blank tube in which all the M-subunit-containing isoenzymes have been removed by a specific immunoprecipitation step. The assay is convenient, linear, and reproducible, and results compare well with those by agarose electrophoresis.This publication has 15 references indexed in Scilit:
- Further observations on the incidence and nature of atypical creatine kinase activity.Clinical Chemistry, 1979
- Macro creatine kinase BB in serum, and some data on its prevalence.Clinical Chemistry, 1979
- Atypical Serum Creatine Kinase Isoenzyme Pattern Caused by Complexing of Creatine Kinase-BB with Immunoglobulins G and Acclm, 1979
- Creatine kinase isoenzyme variants in human serum.Clinical Chemistry, 1978
- Radioimmunoassay of creatine kinase isoenzymes in human serum: isoenzyme BB.Clinical Chemistry, 1978
- Creatine kinase B-subunit activity in human serum. I. Development of an immunoinhibition method for routine determination of S-creatine kinase B-sununit activityClinica Chimica Acta; International Journal of Clinical Chemistry, 1977
- Determination of creatine kinase isoenzyme MB activity in serum using immunological inhibition of creatine kinase M subunit activity activity kinetics and diagnostic significance in myocardial infarctionClinica Chimica Acta; International Journal of Clinical Chemistry, 1976
- Creatine kinase in serum: 2. Interference of adenylate kinase with the assay.Clinical Chemistry, 1976
- The enzyme diagnosis of myocardial infarctionHuman Pathology, 1975
- AN IMPROVED PROCEDURE FOR SERUM CREATINE PHOSPHOKINASE DETERMINATION1967