Cleavage of Adenovirus Type 2 DNA into Six Unique Fragments by Endonuclease R·RI
- 1 January 1973
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 70 (1) , 200-204
- https://doi.org/10.1073/pnas.70.1.200
Abstract
The DNA of adenovirus type 2 was cleaved by restriction endonuclease R.RI into six fragments. These fragments were separated by electrophoresis on composite agarose-polyacrylamide gels. Their molecular weights ranged from 1.1 x 10(6) to 13.6 x 10(6), as measured by electron microscopy. Each fragment represented a unique segment of adenovirus type 2 DNA since: (i) the fragments were obtained in equimolar amounts; (ii) the sum of their molecular weights was equal to the molecular weight of complete adenovirus DNA; and (iii) each fragment exhibited a rate of renaturation that was inversely proportional to its size.Keywords
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