Endonucleases for UV-irradiated and depurinated DNA in barley chloroplasts
Open Access
- 25 March 1980
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 8 (6) , 1373-1382
- https://doi.org/10.1093/nar/8.6.1373
Abstract
Lysates of barley chloroplasts release more radioactivity into acid soluble form from UV-irradiated and alkylated-depurinated E. coli [3H] DNA than from intact DNA. By means of affinity chromatography on depurinated DNA-cellulose and/or UV irradiated DNA-cellulose and by electrophoresis in polyacrylamide gels, four activities on depurinated DNA were separated. One of these contained activity against heavily UV-irradiated /270 J.m−2/ native DNA. In addition, two other nucleases specific towards UV-DNA were separated. One of them was active on native and heat denatured DNA irradiated with 10 J . m−2 UV, whereas the other was predominantly active on native UV-irradiated DNA.Keywords
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