Separation and Quantitation of O-Phthalaldehyde Derivatives of Taurine and Related Compounds in a High Performance Liquid Chromatography (HPLC) System

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Abstract
A sensitive specific assay for taurine using high performance liquid chromatograpy and fluorescence measurement is described. The method employs precolumn derivatization with o-phthalaldehyde in the presence of ethanethiol. Taurine is clearly separated from other amino acids including its precursors hypotaurine and cysteine sulfinic acid. The fluorescence peak height is linear between 1 and 100 picomoles of taurine. There is clear separation of taurine from a contaminant of other taurine assays, α-glycerophosphoryl ethanolamine.