Response of Some Blood Constituents to Infusions of Disodium Ethylenediamine Tetraacetate in Intact Cattle

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Abstract
Lactating and dry cows were intravenously infused with a 10% aqueous solution of disodium ethylenediamine tetraacetate. Infusion was at a constant rate until tetany developed. Blood samples were drawn before infusion and at specified times during and post-infusion. The serum was analyzed for Ca and inorganic P. Total leucocytes were counted. Smears were made from each blood sample and stained for differential counts. The means are from 19 observations (13 for the leucocytes). The serum Ca mean preinfusion level was 10.79mg per 100ml, decreasing to a mean of 4.9mg per 100ml at tetany. Tetany occurred at a mean of 163 min from the beginning of infusion. At 24-hour post-infusion the mean was l0.11mg/l00ml. The mean preinfusion level of P was 6.10mg/100ml and the mean level at tetany was 4.28mg/100ml. The low mean of P, 3.89mg/100ml, occurred 90 min post-tetany. The preinfusion levels of P were practically attained by 12-hr post-infusion. A slight decrease followed by a steady increase to a peak at 8-hr post-infusion occurred in the total white blood cell count. There was a gradual decrease in number from the peak to the 24-hr sample. A sharp diminution in the per cent of the neutrophils caused a crossing over in the percentages of these types of cells by the 3-hr post-infusion sampling. Normal ratios had not been attained by 24-hr post-infusion.