Site-directed mutagenesis of a tRNA gene: base alterations in the coding region affect transcription.
- 1 March 1982
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 79 (5) , 1388-1392
- https://doi.org/10.1073/pnas.79.5.1388
Abstract
Point mutations were introduced in vitro in a cloned nematode tRNAPro gene. Four different mutant clones altered in the DNA that codes for tRNAPro were isolated. Studies on the expression of the mutant genes by microinjection into Xenopus oocyte nuclei reveal that their activities as transcription templates are reduced. DNA sequences that code for the extra arm and the stem of the T-.PSI.-C-G arm of the tRNAPro have an important role in the initiation of tRNA gene transcription.This publication has 24 references indexed in Scilit:
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