Heterogeneity of poly(I).poly(C)-induced human fibroblast interferon mRNA species
- 1 November 1980
- journal article
- research article
- Published by Springer Nature in Nature
- Vol. 288 (5786) , 95-97
- https://doi.org/10.1038/288095a0
Abstract
Since it is generally considered that fibroblast (F) IFN (interferon) represents a single protein, current efforts to clone human F IFN mRNA sequences are based on the observation that F IFN mRNA sediments in sucrose gradients as a single RNA species of size corresponding to 12-14 S. However, gel electrophoresis of mRNA from human fibroblast FS-4 cells exposed to poly(I.cntdot.C) and cycloheximide showed that 2 populations of translationally active human F IFN mRNA molecules exist.sbd.an abundant 14 S species and a scarce 11 S species. Microinjection of either species of mRNA into Xenopus oocytes led to the synthesis of biologically active F-type human IFN.Keywords
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