An Os-1 family histidine kinase from a filamentous fungus confers fungicide-sensitivity to yeast
- 18 March 2005
- journal article
- Published by Springer Nature in Current Genetics
- Vol. 47 (5) , 298-306
- https://doi.org/10.1007/s00294-005-0572-6
Abstract
Three groups of fungicides (phenylpyrroles, dicarboximides, aromatic hydrocarbons) are effective against filamentous fungi. The target of these fungicides is the osmotic stress signal transduction pathway, which is dependent on the Os-1 family of two-component histidine kinases. These fungicides usually have no fungicidal effect on the yeast Saccharomyces cerevisiae. In this report, we found that expression of Hik1, an Os-1 orthologue from rice blast fungus, can confer fungicide-sensitivity to yeast. This requires both the histidine kinase and the response regulator domains of Hik1. Analysis of yeast mutants indicated that this sensitivity is Hog1- and Ssk1-dependent. In addition, our studies revealed an interaction between Hik1 and Ypd1. These observations suggest that Hik1 is a direct target of the fungicides or is a mediator of fungicide action and that the fungicidal effect is transmitted to the Hog1 pathway via Ypd1.Keywords
This publication has 33 references indexed in Scilit:
- Cloning and characterization of the histidine kinase gene Dic1 from Cochliobolus heterostrophus that confers dicarboximide resistance and osmotic adaptationMolecular Genetics and Genomics, 2004
- Whole-Genome Analysis of Two-Component Signal Transduction Genes in Fungal PathogensEukaryotic Cell, 2003
- Yeast osmosensor Sln1 and plant cytokinin receptor Cre1 respond to changes in turgor pressureThe Journal of cell biology, 2003
- Isolation and Functional Analysis of a Gene, tcsB , Encoding a Transmembrane Hybrid-Type Histidine Kinase from Aspergillus nidulansApplied and Environmental Microbiology, 2002
- Molecular Dissection of Alleles of the osmotic-1 Locus of Neurospora crassaFungal Genetics and Biology, 2002
- Characterization of mutations in the two‐component histidine kinase gene that confer fludioxonil resistance and osmotic sensitivity in the os‐1 mutants of Neurospora crassaPest Management Science, 2001
- Identification of CRE1 as a cytokinin receptor from ArabidopsisNature, 2001
- Sensitivity to Phenylpyrrole Fungicides and Abnormal Glycerol Accumulation in Os and Cut Mutant Strains of Neurospora crassaJournal of Pesticide Science, 2000
- Independent Signaling Pathways Regulate Cellular Turgor during Hyperosmotic Stress and Appressorium-Mediated Plant Infection by Magnaporthe griseaPlant Cell, 1999
- Yeast HOG1 MAP Kinase Cascade Is Regulated by a Multistep Phosphorelay Mechanism in the SLN1–YPD1–SSK1 “Two-Component” OsmosensorCell, 1996