A CYTOLOGICAL STUDY OF A PLEOMORPHIC STRAIN OF AZOTOBACTER WITH THE ELECTRON AND PHASE MICROSCOPES AND THE ROBINOW NUCLEAR-STAINING TECHNIQUE

Publisher Website
Google Scholar
Abstract
The form and structure of the cells of A. agile were studied with electron mi-croscopy (RCA model EMU), phase microscopy (Bausch and Lomb and Spencer), and Robinow nuclear-staining. The last method consists of fixation in osmic acid vapor, hydrolysis in [image] HC1, and staining with dilute Giemsa. Using these methods, 4 distinct morphological cell types were observed during the growth of this organism. These types consisted of large plump rods, small rods, elongated and branched forms, and minute coccoid cells. Deep-staining intracellular bodies were observed in young cells, which suggest they may represent structures similar to the "chromotinic bodies" which have been described in other bacterial spp.

This publication has 10 references indexed in Scilit: