O‐Glycosylation of G‐protein‐coupled receptor, octopus rhodopsin
- 4 May 2001
- journal article
- Published by Wiley in FEBS Letters
- Vol. 496 (1) , 19-24
- https://doi.org/10.1016/s0014-5793(01)02392-4
Abstract
In addition to the N-glycan that is evidently conserved in G-protein-coupled receptors (GPCRs), O-glycans in the N-terminus of GPCRs have been suggested. Using a combination of enzymatic and manual Edman degradation in conjunction with G-protein coupled receptor mass spectrometry, the structure and sites of O-glycans in octopus rhodopsin are determined. Two N-acetylgalactosamine residues are O-linked to Thr4 and Thr5 in the N-terminus of octopus rhodopsin. Further, we found chicken iodopsin, but not bovine rhodopsin, contains N-acetylgalactosamine. This is the first direct evidence to determine the structure and sites of O-glycans in GPCRs.Keywords
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