Polynucleotides. XLII. Limited addition of 2'-O-o-nitrobenzyl nucleotides to the 3'-end of ribooligonucleotide with polynucleotide phosphorylase

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Abstract
2′-O-o-Nitrobenzyluridine, -cytidine and -adenosine were phosphorylated with phosphoryl chloride to the corresponding 5′-phosphates and led to 5′-diphosphates by the method of Moffatt and Khorana. These 2′-O-oNB-nucleoside 5′-diphosphates were incubated with a primer CpApA and polynucleotide phosphorylase in the presence of Mn2+. Tetranucleotides CpApApU, CpApApC and CpApApA were obtained after photosensitive removal of oNB groups in yields of 54–70%.