Groα-mediated recruitment of neutrophils is required for elicitation of contact hypersensitivity

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Abstract
The factors mediating recruitment of immune T cells to challenge sites during contact hypersensitivity (CHS) responses remain unclear. To investigate the role of chemokines during elicitation of CHS, the temporal expression of chemokine genes in hapten‐challenged ears was tested. KC (the murine homologoue of Groα) was expressed 30 min following hapten challenge in naive and hapten‐sensitized mice. A rabbit KC‐specific antiserum inhibited elicitation of CHS when administered to sensitized mice prior to hapten challenge. Injecting either neutrophils or immune CD8+ T cells into the ear tissue of immune animals before hapten challenge circumvented the KC antiserum‐mediated inhibition of CHS. Neutrophil depletion also inhibited CHS and was circumvented by injecting either neutrophils or hapten‐primed CD8+ T cells into ears of sensitized mice followed by specific hapten challenge. These results indicate that KC‐directed neutrophil infiltration of hapten challenge sites is required for elicitation of CHS and suggest that neutrophils mediate recruitment of the hapten‐specific CD8+ T cells that subsequently produce cytokines mediating the hypersensitivity response.

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