Effects of gastrin and vagal denervation on DNA synthesis in canine fundic mucosa.

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Abstract
The doses of histamine and gastrin 17 II producing half-maximal acid secretion were determined in 3 dogs with gastric fistulas and vagally denervated (Heidenhain) pouches. After a 24 h fast, either saline, histamine (D50 = 24 .mu.g/kg .cntdot. h) or gastrin (D50 = 160 ng/kg .cntdot. h) was infused i.v. for 4 h. The rate of mucosal DNA synthesis was measured in biopsies obtained from both the pouch and main stomach at 4 h intervals for 24 h from the beginning of secretogogue infusion. In the resting state, DNA synthesis was 75% higher in denervated than innervated oxyntic gland mucosa. After gastrin infusion and after the physiological stimulus of a meat meal, significant increases in DNA synthesis were observed in both the main stomach and pouch compared to saline and histamine controls, the greater response occurring in the vagally denervated mucosa. Serum gastrin levels after feeding were comparable to those during gastrin infusion. Vagally denervated mucosa has a higher rate of DNA synthesis than innervated mucosa. DNA synthesis in denervated mucosa is more responsive to gastrin in the dose used. Physiological amounts of gastrin stimulate DNA synthesis in canine fundic mucosa; The effects of histamine are not significantly different from those of saline.