Differential chronology of TCRADV2 gene use by α and δ chains of the mouse TCR

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Abstract
The genes coding for TCR α and δ chains share the same genetic locus (TCRA/D). The rules governing the utilization of a V gene with the α and δ chains have not been established. More specifically, it is not known whether the position of a gene within the locus influences its utilization in α and δ TCR. To elucidate these points, we mapped ADV2 genes in the TCRA/D locus of BALB/c mice and analyzed their utilization in TCR α and δ transcripts from thymi isolated from mice of different ages. Our results show that all ADV2 genes can be used by the two chains, but with strikingly different patterns. Moreover, ADV2 utilization by the α chain proceeds in successive concentric waves during development, suggesting a progressive regulation of gene accessibility and utilization. These results support independent control of TCRA and TCRD gene assembly.