Luminescent Silica Nanobeads: Characterization and Evaluation as Efficient Cytoplasmatic Transporters for T-Lymphocytes
- 1 June 2007
- journal article
- research article
- Published by American Chemical Society (ACS) in Journal of the American Chemical Society
- Vol. 129 (25) , 7814-7823
- https://doi.org/10.1021/ja070245c
Abstract
We report the fabrication and characterization of neutravidin-conjugated silica nanobeads doped with a ruthenium-complex luminophore and functionalized with antihuman CD3, antihuman CD28, and an acid-sensitive polymer. We observed that the nanobeads were readily delivered into Jurkat T leukemia cells by endocytosis, transported into lysosomes and subsequently into the cytoplasm as revealed by pH-sensitive luminescence. Since signs of cytotoxicity were not observed, the reported nanobeads could be an excellent and nontoxic building block for efficient intracellular transporters.Keywords
This publication has 58 references indexed in Scilit:
- The emerging nanomedicine landscapeNature Biotechnology, 2006
- Full-Length Single-Walled Carbon Nanotubes Decorated with Streptavidin-Conjugated Quantum Dots as Multivalent Intracellular Fluorescent NanoprobesBiomacromolecules, 2006
- Self-Assembled Quantum Dot−Peptide Bioconjugates for Selective Intracellular DeliveryBioconjugate Chemistry, 2006
- Surface Modification of Silica Nanoparticles to Reduce Aggregation and Nonspecific BindingLangmuir, 2006
- Medical application of functionalized magnetic nanoparticlesJournal of Bioscience and Bioengineering, 2005
- Highly efficient molecular delivery into mammalian cells using carbon nanotube spearingNature Methods, 2005
- Novel fluorescent silica nanoparticle probe for ultrasensitive immunoassaysAnalytica Chimica Acta, 2003
- Radiationless Relaxation in a Synthetic Analogue of the Green Fluorescent Protein ChromophoreThe Journal of Physical Chemistry B, 2001
- Synthesis and Properties of Biocompatible Water-Soluble Silica-Coated CdSe/ZnS Semiconductor Quantum DotsThe Journal of Physical Chemistry B, 2001
- Biotin binding changes the conformation and decreases tryptophan accessibility of streptavidinProtein Journal, 1990